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《The Plant Cell》: 水稻理想株型研究獲新進展 
作者:中國水稻信息網
來源:中國水稻信息網
發布時間:2013-11-11

日前,The Plant Cell在線發表了我國科學家研究水稻理想株型而獲得的最新科研進展。

水稻的株型是決定水稻産量的主要因素之一,水稻理想株型的塑造是提高水稻産量的重要途徑。控制水稻理想株型的主基因IPA1 (Ideal Plant Architecture 1) 編碼一個含SBP-box的轉錄因子,參與調控多個生長發育過程。研究人員最近的研究發現,IPA1作爲轉錄激活因子起作用,通過全基因組染色質免疫共沉澱-測序分析分別確定了水稻莖尖和幼穗含有IPA1結合位點相關的一系列基因,證明IPA1蛋白可以通過SBP-box結構域直接與受調控基因的核心基序GTAC相結合,也可以通過與TCP家族的轉錄因子PCF1和PCF2相互作用與TGGGCC/T基序間接相結合。通過RNA測序對全基因組表達譜進一步分析顯示IPA1參與調控多個生長發育過程。此外,該研究還表明,IPA1可以通過TB1調控水稻分蘖,通過DEP1調控水稻的株高和穗長。該研究爲進一步解析水稻株型調控遺傳調控網絡奠定了基礎。

根據2013年ISI發布的JCR(Journal Citation Reports)數據,The Plant Cell最新SCI影響因子爲9.251;過去的12年間,該刊物影響因子處于8.987至11.295之間。根據《2012 JCR Science Edition》數據,該刊最新的學科排名分別爲:在總計197種期刊的植物學(PLANT SCIENCES)中排名第4位; 總計290種期刊的生物化學與分子生物學(BIOCHEMISTRY & MOLECULAR BIOLOGY)中排名第21位;總計185種期刊的細胞生物學(CELL BIOLOGY)中排名第23位。

論文摘要:

IDEAL PLANT ARCHITECTURE1 (IPA1) is critical in regulating rice (Oryza sativa) plant architecture and substantially enhances grain yield. To elucidate its molecular basis, we first confirmed IPA1 as a functional transcription activator and then identified 1067 and 2185 genes associated with IPA1 binding sites in shoot apices and young panicles, respectively, through chromatin immunoprecipitation sequencing assays. The SQUAMOSA PROMOTER BINDING PROTEIN-box direct binding core motif GTAC was highly enriched in IPA1 binding peaks; interestingly, a previously uncharacterized indirect binding motif TGGGCC/T was found to be significantly enriched through the interaction of IPA1 with proliferating cell nuclear antigen PROMOTER BINDING FACTOR1 or PROMOTER BINDING FACTOR2. Genome-wide expression profiling by RNA sequencing revealed IPA1 roles in diverse pathways. Moreover, our results demonstrated that IPA1 could directly bind to the promoter of rice TEOSINTE BRANCHED1, a negative regulator of tiller bud outgrowth, to suppress rice tillering, and directly and positively regulate DENSE AND ERECT PANICLE1, an important gene regulating panicle architecture, to influence plant height and panicle length. The elucidation of target genes of IPA1 genome-wide will contribute to understanding the molecular mechanisms underlying plant architecture and to facilitating the breeding of elite varieties with ideal plant architecture.

詳細信息:

http://www.cnrri.cn/zjww/Detail.aspx?id=20024572

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上篇文章:《Nature Communications》:揭示水稻結實率關鍵控制基因
下篇文章:《Plant Physiology》:發現水稻受體類激酶OsSIK2提高耐鹽性並延緩衰老
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